Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action

This paper introduces the MIX-Seq method for multiplexed transcriptional profiling of pooled cell lines that undergo drug treatment. Individual cell line identities were determined based on their single-nucleotide polymorphism (SNP) profiles. This method enables profiling of chemical or genetic perturbation response in pools of 100+ cancer cell lines.